A fast cross-validation method for alignment of electron tomography images based on Beer-Lambert law.

ABSTRACT: In electron tomography, accurate alignment of tilt series is an essential step in attaining high-resolution 3D reconstructions. Nevertheless, quantitative assessment of alignment quality has remained a challenging issue, even though many alignment methods have been reported. Here, we report a fast and accurate method, tomoAlignEval, based on the Beer-Lambert law, for the evaluation of alignment quality. Our method is able to globally estimate the alignment accuracy by measuring the goodness of log-linear relationship of the beam intensity attenuations at different tilt angles. Extensive tests with experimental data demonstrated its robust performance with stained and cryo samples. Our method is not only significantly faster but also more sensitive than measurements of tomogram resolution u

SUBMITTER: Yan R 

PROVIDER: S-EPMC4633358 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature