The ubiquitin ligase Siah2 regulates obesity-induced adipose tissue inflammation.

ABSTRACT:

Objective

Chronic, low-grade adipose tissue inflammation associated with adipocyte hypertrophy is an important link in the relationship between obesity and insulin resistance. Although ubiquitin ligases regulate inflammatory processes, the role of these enzymes in metabolically driven adipose tissue inflammation is relatively unexplored. Herein, the effect of the ubiquitin ligase Siah2 on obesity-related adipose tissue inflammation was examined.

Methods

Wild-type and Siah2KO mice were fed a low- or high-fat diet for 16 weeks. Indirect calorimetry, body composition, and glucose and insulin tolerance were assayed along with glucose and insulin levels. Gene and protein expression, immunohistochemistry, adipocyte size distribution, and lipolysis were also analyzed.

Results

SUBMITTER: Kilroy G 

PROVIDER: S-EPMC4633373 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature