Automated batch fiducial-less tilt-series alignment in Appion using Protomo.

ABSTRACT: The field of electron tomography has benefited greatly from manual and semi-automated approaches to marker-based tilt-series alignment that have allowed for the structural determination of multitudes of in situ cellular structures as well as macromolecular structures of individual protein complexes. The emergence of complementary metal-oxide semiconductor detectors capable of detecting individual electrons has enabled the collection of low dose, high contrast images, opening the door for reliable correlation-based tilt-series alignment. Here we present a set of automated, correlation-based tilt-series alignment, contrast transfer function (CTF) correction, and reconstruction workflows for use in conjunction with the Appion/Leginon package that are primarily targeted at automating structure

SUBMITTER: Noble AJ 

PROVIDER: S-EPMC4633401 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature