PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation.

ABSTRACT: Alternative splicing of nuclear pre-mRNA is essential for generating protein diversity and regulating gene expression. While many immunologically relevant genes undergo alternative splicing, the role of regulated splicing in T cell immune responses is largely unexplored, and the signaling pathways and splicing factors that regulate alternative splicing in T cells are poorly defined. Here, we show using a combination of Jurkat T cells, human primary T cells, and ex vivo naïve and effector virus-specific T cells isolated after influenza A virus infection that SC35 phosphorylation is induced in response to stimulatory signals. We show that SC35 colocalizes with RNA polymerase II in activated T cells and spatially overlaps with H3K27ac and H3K4me3, which mark transcriptionally active genes. In

SUBMITTER: McCuaig RD 

PROVIDER: S-EPMC4633479 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature