Screening and Molecular Analysis of Single Circulating Tumor Cells Using Micromagnet Array.

ABSTRACT: Immunomagnetic assay has been developed to detect rare circulating tumor cells (CTCs), which shows clinical significance in cancer diagnosis and prognosis. The generation and fine-tuning of the magnetic field play essential roles in such assay toward effective single-cell-based analyses of target cells. However, the current assay has a limited range of field gradient, potentially leading to aggregation of cells and nanoparticles. Consequently, quenching of the fluorescence signal and mechanical damage to the cells may occur, which lower the system sensitivity and specificity. We develop a micromagnet-integrated microfluidic system for enhanced CTC detection. The ferromagnetic micromagnets, after being magnetized, generate localized magnetic field up to 8-fold stronger than that without the

SUBMITTER: Huang YY 

PROVIDER: S-EPMC4633592 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature