Transferrin-bound proteins as potential biomarkers for advanced breast cancer patients.

ABSTRACT:

Background

Serum profiling using mass spectrometry-based proteomic techniques has great potential to detect biomarkers that might improve the management for advanced breast cancer patients. The albuminome has previously been investigated as a tool in biomarker discovery, however other high abundant blood proteins are also likely to sequester potentially interesting molecules.

Methods

Affinity resin purified and isolated Transferrin and associated bound proteins from normal control and breast cancer patient serum samples were analysed by label-free mass spectrometry during the discovery phase.

Results

21 significant proteins were identified with Fibrinogen and Fibronectin selected for further analysis in an independent sample set, with significant difference found when

SUBMITTER: Dowling P 

PROVIDER: S-EPMC4633920 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature