Transcriptome, microRNA, and degradome analyses of the gene expression of Paulownia with phytoplamsa.

ABSTRACT:

Background

Paulownia witches' broom (PaWB) is a fatal disease of Paulownia caused by a phytoplasma. In previous studies, we found that plants with PaWB symptoms would revert to a healthy morphology after methyl methane sulfonate (MMS) treatment. To completely understand the gene expression profiles of the Paulownia-phytoplasma interaction, three high-throughput sequencing technologies were used to investigate changes of gene expression and microRNAs (miRNAs) in healthy Paulownia tomentosa plantlets, PaWB-infected plantlets, and PaWB-infected plantlets treated with 60 mg · L(-1) MMS.

Methods

Transcriptome, miRNAs and degradome sequencing were performed to explore the global gene expression profiles in the process of Paulownia tomentosa with phytoplasma infection.

Results</

SUBMITTER: Fan G 

PROVIDER: S-EPMC4634154 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature