Evidence-Based Structural Model of the Staphylococcal Repressor Protein: Separation of Functions into Different Domains.

ABSTRACT: Horizontal transfer of mobile genetic elements within Staphylococci is of high biomedical significance as such elements are frequently responsible for virulence and toxic effects. Staphylococcus-encoded repressor proteins regulate the replication of these mobile genetic elements that are located within the so-called pathogenicity islands. Here, we report structural and functional characterization of one such repressor protein, namely the Stl protein encoded by the pathogenicity island SaPIbov1. We create a 3D structural model and based on this prediction, we investigate the different functionalities of truncated and point mutant constructs. Results suggest that a helix-turn-helix motif governs the interaction of the Stl protein with its cognate DNA site: point mutations within this motif d

SUBMITTER: Nyiri K 

PROVIDER: S-EPMC4634304 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature