Proteomic analysis of nuclear factors binding to an intronic enhancer in the myelin proteolipid protein gene.

ABSTRACT: The myelin proteolipid protein gene (Plp1) encodes the most abundant protein found in CNS myelin, accounting for nearly one-half of the total protein. Its expression in oligodendrocytes is developmentally regulated - peaking during the active myelination period of CNS development. Previously, we have identified a novel enhancer (designated ASE) in intron 1 DNA that appears to be important in mediating the surge of Plp1 gene activity during the active myelination period. Evidence suggests that the ASE participates in the formation of a specialized multi-protein/DNA complex called an enhanceosome. The current study describes an optimized, five-step, DNA affinity chromatography purification procedure to purify nuclear proteins from mouse brain that bind to the 85-bp ASE sequence, specifically

SUBMITTER: Dobretsova A 

PROVIDER: S-EPMC4634561 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature