Analysis of the FnrL regulon in Rhodobacter capsulatus reveals limited regulon overlap with orthologues from Rhodobacter sphaeroides and Escherichia coli.

ABSTRACT:

Background

FNR homologues constitute an important class of transcription factors that control a wide range of anaerobic physiological functions in a number of bacterial species. Since FNR homologues are some of the most pervasive transcription factors, an understanding of their involvement in regulating anaerobic gene expression in different species sheds light on evolutionary similarity and differences. To address this question, we used a combination of high throughput RNA-Seq and ChIP-Seq analysis to define the extent of the FnrL regulon in Rhodobacter capsulatus and related our results to that of FnrL in Rhodobacter sphaeroides and FNR in Escherichia coli.

Results

Our RNA-seq results show that FnrL affects the expression of 807 genes, which accounts for over 20 % of the R

SUBMITTER: Kumka JE 

PROVIDER: S-EPMC4634722 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature