Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines.

ABSTRACT: Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutin

SUBMITTER: Huang D 

PROVIDER: S-EPMC4634975 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature