Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice.

ABSTRACT: During attempts to clone retroviral determinants associated with a mouse model of Langerhans cell histiocytosis (LCH), suppression subtractive hybridization (SSH) was used to identify unique viruses in the liver of severe combined immunodeficiency (SCID) mice transplanted with LCH tissues. A partial genomic sequence of a murine coronavirus was identified, and the whole genome (31428 bp) of the coronavirus was subsequently sequenced using PCR cloning techniques. Nucleotide sequence comparisons revealed that the genome sequence of the new virus was 91-93% identical to those of known murine hepatitis viruses (MHVs). The predicted open reading frame from the nucleotide sequence encoded all known proteins of MHVs. Analysis at the protein level showed that the virus was closely related to the hi

SUBMITTER: Islam MM 

PROVIDER: S-EPMC4635179 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature