Identification of a novel cell culture adaptation site on the capsid of foot-and-mouth disease virus.

ABSTRACT: Vaccination remains the most effective tool for control of foot-and-mouth disease both in endemic countries and as an emergency preparedness for new outbreaks. Foot-and-mouth disease vaccines are chemically inactivated virus preparations and the production of new vaccines is critically dependent upon cell culture adaptation of field viruses, which can prove problematic. A major driver of cell culture adaptation is receptor availability. Field isolates of foot-and-mouth disease virus (FMDV) use RGD-dependent integrins as receptors, whereas cell culture adaptation often selects for variants with altered receptor preferences. Previously, two independent sites on the capsid have been identified where mutations are associated with improved cell culture growth. One is a shallow depression formed

SUBMITTER: Chamberlain K 

PROVIDER: S-EPMC4635497 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature