Complete haplotype phasing of the MHC and KIR loci with targeted HaploSeq.

ABSTRACT:

Background

The MHC and KIR loci are clinically relevant regions of the genome. Typing the sequence of these loci has a wide range of applications including organ transplantation, drug discovery, pharmacogenomics and furthering fundamental research in immune genetics. Rapid advances in biochemical and next-generation sequencing (NGS) technologies have enabled several strategies for precise genotyping and phasing of candidate HLA alleles. Nonetheless, as typing of candidate HLA alleles alone reveals limited aspects of the genetics of MHC region, it is insufficient for the comprehensive utility of the aforementioned applications. For this reason, we believe phasing the entire MHC and KIR locus onto a single locus-spanning haplotype can be a critical improvement for better understandin

SUBMITTER: Selvaraj S 

PROVIDER: S-EPMC4636068 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature