Deciphering Transcriptional Dynamics In Vivo by Counting Nascent RNA Molecules.

ABSTRACT: Deciphering how the regulatory DNA sequence of a gene dictates its expression in response to intra and extracellular cues is one of the leading challenges in modern genomics. The development of novel single-cell sequencing and imaging techniques, as well as a better exploitation of currently available single-molecule imaging techniques, provides an avenue to interrogate the process of transcription and its dynamics in cells by quantifying the number of RNA polymerases engaged in the transcription of a gene (or equivalently the number of nascent RNAs) at a given moment in time. In this paper, we propose that measurements of the cell-to-cell variability in the number of nascent RNAs provide a mostly unexplored method for deciphering mechanisms of transcription initiation in cells. We propose

SUBMITTER: Choubey S 

PROVIDER: S-EPMC4636183 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature