Transcriptome-wide analysis supports environmental adaptations of two Pinus pinaster populations from contrasting habitats.

ABSTRACT:

Background

Maritime pine (Pinus pinaster Aiton) grows in a range of different climates in the southwestern Mediterranean region and the existence of a variety of latitudinal ecotypes or provenances is well established. In this study, we have conducted a deep analysis of the transcriptome in needles from two P. pinaster provenances, Leiria (Portugal) and Tamrabta (Morocco), which were grown in northern Spain under the same conditions.

Results

An oligonucleotide microarray (PINARRAY3) and RNA-Seq were used for whole-transcriptome analyses, and we found that 90.95% of the data were concordant between the two platforms. Furthermore, the two methods identified very similar percentages of differentially expressed genes with values of 5.5% for PINARRAY3 and 5.7% for RNA-Seq. In tot

SUBMITTER: Canas RA 

PROVIDER: S-EPMC4636790 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature