Theoretical limitations of quantification for noncompetitive sandwich immunoassays.

ABSTRACT: Immunoassays exploit the highly selective interaction between antibodies and antigens to provide a vital method for biomolecule detection at low concentrations. Developers and practitioners of immunoassays have long known that non-specific binding often restricts immunoassay limits of quantification (LOQs). Aside from non-specific binding, most efforts by analytical chemists to reduce the LOQ for these techniques have focused on improving the signal amplification methods and minimizing the limitations of the detection system. However, with detection technology now capable of sensing single-fluorescence molecules, this approach is unlikely to lead to dramatic improvements in the future. Here, fundamental interactions based on the law of mass action are analytically connected to signal gener

SUBMITTER: Woolley CF 

PROVIDER: S-EPMC4636921 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature