T Cell Responses against Mycobacterial Lipids and Proteins Are Poorly Correlated in South African Adolescents.

ABSTRACT: Human T cells are activated by both peptide and nonpeptide Ags produced by Mycobacterium tuberculosis. T cells recognize cell wall lipids bound to CD1 molecules, but effector functions of CD1-reactive T cells have not been systematically assessed in M. tuberculosis-infected humans. It is also not known how these features correlate with T cell responses to secreted protein Ags. We developed a flow cytometric assay to profile CD1-restricted T cells ex vivo and assessed T cell responses to five cell wall lipid Ags in a cross-sectional study of 19 M. tuberculosis-infected and 22 M. tuberculosis-uninfected South African adolescents. We analyzed six T cell functions using a recently developed computational approach for flow cytometry data in high dimensions. We compared these data with T cell re

SUBMITTER: Seshadri C 

PROVIDER: S-EPMC4637215 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature