Multiple Reaction Monitoring Enables Precise Quantification of 97 Proteins in Dried Blood Spots.

ABSTRACT: The dried blood spot (DBS) methodology provides a minimally invasive approach to sample collection and enables room-temperature storage for most analytes. DBS samples have successfully been analyzed by liquid chromatography multiple reaction monitoring mass spectrometry (LC/MRM-MS) to quantify a large range of small molecule biomarkers and drugs; however, this strategy has only recently been explored for MS-based proteomics applications. Here we report the development of a highly multiplexed MRM assay to quantify endogenous proteins in human DBS samples. This assay uses matching stable isotope-labeled standard peptides for precise, relative quantification, and standard curves to characterize the analytical performance. A total of 169 peptides, corresponding to 97 proteins, were quantified

SUBMITTER: Chambers AG 

PROVIDER: S-EPMC4638049 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature