Intramedullary megakaryocytes internalize released platelet factor 4 and store it in alpha granules.

ABSTRACT:

Background

Megakaryocytes express and store platelet factor 4 (PF4) in alpha granules. In vivo, PF4 is a clinically relevant, negative regulator of megakaryopoiesis and hematopoietic stem cell replication. These findings would suggest a regulated source of free intramedullary PF4.

Objectives

Define the source of free intramedullary PF4 and its intramedullary life cycle.

Methods

We interrogated both murine and human bone marrow-derived cells during megakaryopoiesis in vitro by using confocal microscopy and enzyme-linked immunosorbent assay. With immunohistochemistry, we examined in vivo free PF4 in murine bone marrow before and after radiation injury and in the setting of megakaryocytopenia and thrombocytopenia.

Results

Exogenously added human PF4 is internalize

SUBMITTER: Lambert MP 

PROVIDER: S-EPMC4638179 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature