Ceramide changes the mediator of flow-induced vasodilation from nitric oxide to hydrogen peroxide in the human microcirculation.

ABSTRACT:

Rationale

Mitochondrial-derived hydrogen peroxide (H2O2) regulates flow-induced dilation (FID) in microvessels from patients with coronary artery disease. The relationship between ceramide, an independent risk factor for coronary artery disease and a known inducer of mitochondrial reactive oxygen species, and FID is unknown.

Objective

We examined the hypothesis that exogenous ceramide induces a switch in the mediator of FID from nitric oxide to H2O2.

Methods and results

Internal diameter changes of resistance arterioles from human adipose and atrial tissue were measured by video microscopy. Mitochondrial H2O2 production was assayed in arterioles using mito peroxy yellow 1. Polyethylene glycol-catalase, rotenone, and Mito-TEMPO impaired FID in healthy adipose arteriole

SUBMITTER: Freed JK 

PROVIDER: S-EPMC4640193 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature