High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs.

ABSTRACT: MicroRNAs (miRNAs) are non-coding RNAs of ~22 nucleotides in length that regulate gene expression by interfering with the stability and translation of mRNAs. Their expression is regulated during development, under a wide variety of stress conditions and in several pathological processes. In nature, animals often face feast or famine conditions. We observed that subjecting early L4 larvae from Caenorhabditis elegans to a 12-hr starvation period produced worms that are thinner and shorter than well-fed animals, with a decreased lipid accumulation, diminished progeny, reduced gonad size, and an increased lifespan. Our objective was to identify which of the 302 known miRNAs of C. elegans changed their expression under starvation conditions as compared to well-fed worms by means of deep sequenc

SUBMITTER: Garcia-Segura L 

PROVIDER: S-EPMC4640506 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature