Phosphorylation of the Cav3.2 T-type calcium channel directly regulates its gating properties.

ABSTRACT: Phosphorylation is a major mechanism regulating the activity of ion channels that remains poorly understood with respect to T-type calcium channels (Cav3). These channels are low voltage-activated calcium channels that play a key role in cellular excitability and various physiological functions. Their dysfunction has been linked to several neurological disorders, including absence epilepsy and neuropathic pain. Recent studies have revealed that T-type channels are modulated by a variety of serine/threonine protein kinase pathways, which indicates the need for a systematic analysis of T-type channel phosphorylation. Here, we immunopurified Cav3.2 channels from rat brain, and we used high-resolution MS to construct the first, to our knowledge, in vivo phosphorylation map of a voltage-gated c

SUBMITTER: Blesneac I 

PROVIDER: S-EPMC4640759 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature