Validation of a HPLC/FLD Method for Quantification of Tocotrienols in Human Plasma.

ABSTRACT: Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in human plasma. Using both the external and internal standards, tocotrienol homologues were quantified via a normal-phase high performance liquid chromatography with fluorescence detector maintained at the excitation wavelength of 295 nm and the emission wavelength of 325 nm. The four tocotrienol homologues were well separated within 30 minutes. A large interindividual variation between subjects was observed as the absorption of tocotrienols is dependent on food matrix and gut lipolysis. The accurac

SUBMITTER: Che HL 

PROVIDER: S-EPMC4641197 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature