σ54-dependent regulome in Desulfovibrio vulgaris Hildenborough.

ABSTRACT:

Background

The σ(54) subunit controls a unique class of promoters in bacteria. Such promoters, without exception, require enhancer binding proteins (EBPs) for transcription initiation. Desulfovibrio vulgaris Hildenborough, a model bacterium for sulfate reduction studies, has a high number of EBPs, more than most sequenced bacteria. The cellular processes regulated by many of these EBPs remain unknown.

Results

To characterize the σ(54)-dependent regulome of D. vulgaris Hildenborough, we identified EBP binding motifs and regulated genes by a combination of computational and experimental techniques. These predictions were supported by our reconstruction of σ(54)-dependent promoters by comparative genomics. We reassessed and refined the results of earlier studies on regulation i

SUBMITTER: Kazakov AE 

PROVIDER: S-EPMC4641369 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature