Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy.

ABSTRACT:

Background

Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust mite (HDM) allergy has not been studied in detail.

Objective

To express and purify a recombinant Der p 10 with equivalent IgE reactivity as natural Der p 10 and to evaluate its IgE reactivity and allergenic activity in HDM-allergic patients.

Methods

rDer p 10 was expressed in Escherichia coli, purified and characterized by mass spectrometry and circular dichroism. It was tested for IgE reactivity in 1322 HDM-allergic patients. Detailed IgE-reactivity profiles to six HDM allergens (Der p 1, 2, 5, 7, 10, 21) were established for subgroups of Der p 10-positive and -negative patients. The allergenic activity of rDer p 10 was evaluated in basoph

SUBMITTER: Resch Y 

PROVIDER: S-EPMC4642339 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature