RNA sequencing provides exquisite insight into the manipulation of the alveolar macrophage by tubercle bacilli.

ABSTRACT: Mycobacterium bovis, the agent of bovine tuberculosis, causes an estimated $3 billion annual losses to global agriculture due, in part, to the limitations of current diagnostics. Development of next-generation diagnostics requires a greater understanding of the interaction between the pathogen and the bovine host. Therefore, to explore the early response of the alveolar macrophage to infection, we report the first application of RNA-sequencing to define, in exquisite detail, the transcriptomes of M. bovis-infected and non-infected alveolar macrophages from ten calves at 2, 6, 24 and 48 hours post-infection. Differentially expressed sense genes were detected at these time points that revealed enrichment of innate immune signalling functions, and transcriptional suppression of host defence m

SUBMITTER: Nalpas NC 

PROVIDER: S-EPMC4642568 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature