A Novel, Real-Time, In Vivo Mouse Retinal Imaging System.

ABSTRACT:

Purpose

To develop an efficient, low-cost instrument for robust real-time imaging of the mouse retina in vivo, and assess system capabilities by evaluating various animal models.

Methods

Following multiple disappointing attempts to visualize the mouse retina during a subretinal injection using commercially available systems, we identified the key limitation to be inadequate illumination due to off axis illumination and poor optical train optimization. Therefore, we designed a paraxial illumination system for Greenough-type stereo dissecting microscope incorporating an optimized optical launch and an efficiently coupled fiber optic delivery system. Excitation and emission filters control spectral bandwidth. A color coupled-charged device (CCD) camera is coupled to the microsc

SUBMITTER: Butler MC 

PROVIDER: S-EPMC4642603 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature