Characterization of secretomes provides evidence for adipose-derived mesenchymal stromal cells subtypes.

ABSTRACT:

Introduction

This study was aimed at deciphering the secretome of adipose-derived mesenchymal stromal cells (ADSCs) cultured in standard and hypoxic conditions to reveal proteins, which may be responsible for regenerative action of these cells.

Methods

Human ADSCs were isolated from 10 healthy donors and cultured for 3-4 passages. Cells were serum deprived and cell purity was assessed using multiple cell surface markers. Conditioned media was collected and analyzed using LC-MS with a focus on characterizing secreted proteins.

Results

Purity of the ADSC assessed as CD90+/CD73+/CD105+/CD45-/CD31- cells was greater than 99 % and viability was greater than 97 %. More than 600 secreted proteins were detected in conditioned media of ADSCs. Of these 100 proteins were common

SUBMITTER: Kalinina N 

PROVIDER: S-EPMC4642680 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature