Automated amplicon design suitable for analysis of DNA variants by melting techniques.

ABSTRACT:

Background

The technological development of DNA analysis has had tremendous development in recent years, and the present deep sequencing techniques present unprecedented opportunities for detailed and high-throughput DNA variant detection. Although DNA sequencing has had an exponential decrease in cost per base pair analyzed, focused and target-specific methods are however still much in use for analysis of DNA variants. With increasing capacity in the analytical procedures, an equal demand in automated amplicon and primer design has emerged.

Results

We have constructed a web-based tool that is able to batch design DNA variant assay suitable for analysis by denaturing gel/capillary electrophoresis and high resolution melting. The tool is developed as a computational workflow

SUBMITTER: Ekstrom PO 

PROVIDER: S-EPMC4642734 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature