RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium.

ABSTRACT: Salmonella enterica serovar Typhimurium is arguably the world's best-understood bacterial pathogen. However, crucial details about the genetic programs used by the bacterium to survive and replicate in macrophages have remained obscure because of the challenge of studying gene expression of intracellular pathogens during infection. Here, we report the use of deep sequencing (RNA-seq) to reveal the transcriptional architecture and gene activity of Salmonella during infection of murine macrophages, providing new insights into the strategies used by the pathogen to survive in a bactericidal immune cell. We characterized 3583 transcriptional start sites that are active within macrophages, and highlight 11 of these as candidates for the delivery of heterologous antigens from Salmonella vaccine

SUBMITTER: Srikumar S 

PROVIDER: S-EPMC4643027 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature