Combined ultra-low input mRNA and whole-genome sequencing of human embryonic stem cells.

ABSTRACT:

Background

Next Generation Sequencing has proven to be an exceptionally powerful tool in the field of genomics and transcriptomics. With recent development it is nowadays possible to analyze ultra-low input sample material down to single cells. Nevertheless, investigating such sample material often limits the analysis to either the genome or transcriptome. We describe here a combined analysis of both types of nucleic acids from the same sample material.

Methods

The method described enables the combined preparation of amplified cDNA as well as amplified whole-genome DNA from an ultra-low input sample material derived from a sub-colony of in-vitro cultivated human embryonic stem cells. cDNA is prepared by the application of oligo-dT coupled magnetic beads for mRNA capture, fir

SUBMITTER: Mertes F 

PROVIDER: S-EPMC4643517 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature