Analysis of Naturally Occurring Resistant Mutations to Hepatitis C Virus NS3 Protease Inhibitors: A Preliminary Study in South of Iran.

ABSTRACT:

Background

Exploring the rate of naturally occurring NS3 protease mutants in HCV infected population is influential in the future therapeutic approaches.

Objectives

This study explored naturally occurring resistant mutations to protease inhibitors in a pilot study.

Patients and methods

We analyzed NS3 gene sequences in 7 HCV infected patients, referred to the central liver center, south of Iran. The protease domain was amplified by PCR followed by product extraction. Amplified NS3 genes were cloned by TA/cloning system. For each patient, clonal-sequencing was performed to improve mutation detection sensitivity. Then, the obtained sequences were compared with the reference sequences and final phylogenic tree was constructed. Afterwards, the sequences were studied to in

SUBMITTER: Afrasiabi M 

PROVIDER: S-EPMC4644313 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature