The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts.

ABSTRACT:

Introduction

Follistatin-like-1 (FSTL1) is a widely expressed secreted protein with diverse but poorly understood functions. Originally described as a pro-inflammatory molecule, it has recently been reported to play a role in signaling pathways that regulate development and homeostasis. Distinctively, FSTL1 harbors within its 3'-UTR the sequence encoding microRNA-198 (miR-198), shown to be inversely regulated relative to FSTL1 expression and to exhibit opposite actions on cellular processes such as cell migration. We sought to investigate the expression of FSTL1 and to assess its interplay with miR-198 in human trophoblasts.

Methods

We used a combination of northern blot analyses, quantitative PCR, small RNA sequencing, western blot and immunohistochemistry to characterize F

SUBMITTER: Mouillet JF 

PROVIDER: S-EPMC4644452 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature