Proteomic analysis of naturally-sourced biological scaffolds.

ABSTRACT: A key challenge to the clinical implementation of decellularized scaffold-based tissue engineering lies in understanding the process of removing cells and immunogenic material from a donor tissue/organ while maintaining the biochemical and biophysical properties of the scaffold that will promote growth of newly seeded cells. Current criteria for evaluating whole organ decellularization are primarily based on nucleic acids, as they are easy to quantify and have been directly correlated to adverse host responses. However, numerous proteins cause immunogenic responses and thus should be measured directly to further understand and quantify the efficacy of decellularization. In addition, there has been increasing appreciation for the role of the various protein components of the extracellular m

SUBMITTER: Li Q 

PROVIDER: S-EPMC4644483 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature