Enhanced Cardiac Regenerative Ability of Stem Cells After Ischemia-Reperfusion Injury: Role of Human CD34+ Cells Deficient in MicroRNA-377.

ABSTRACT:

Background

MicroRNA (miR) dysregulation in the myocardium has been implicated in cardiac remodeling after injury or stress.

Objectives

The aim of this study was to explore the role of miR in human CD34(+) cell (hCD34(+)) dysfunction in vivo after transplantation into the myocardium under ischemia-reperfusion (I-R) conditions.

Methods

In response to inflammatory stimuli, the miR array profile of endothelial progenitor cells was analyzed using a polymerase chain reaction-based miR microarray. miR-377 expression was assessed in myocardial tissue from human patients with heart failure (HF). We investigated the effect of miR-377 inhibition on an hCD34(+) cell angiogenic proteome profile in vitro and on cardiac repair and function after I-R injury in immunodeficient mice.

SUBMITTER: Joladarashi D 

PROVIDER: S-EPMC4644493 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature