Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay.

ABSTRACT:

Background

Amelogenins are the major components of enamel matrix proteins. Enamel matrix derivatives (EMD) can be used in periodontal diseases to regenerate periodontal tissues. The main aim of this study was to evaluate expression of full-length functional recombinant human amelogenin (rhAm) in Iranian lizard Leishmania (I.L.L.) as an alternative eukaryotic expression system.

Methods

Human cDNA encoding a 175-amino acid amelogenin expression cassette was sub cloned into a pLEXSY vector. The construct was transferred into Leishmania cells by electroporation. The protein production was surveyed in the transcription and the translation levels. The expressed protein was purified and some of its biological properties were investigated in comparison to EMD and negative control.

SUBMITTER: Yadegari Z 

PROVIDER: S-EPMC4645770 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature