Corneal Fibroblast Migration Patterns During Intrastromal Wound Healing Correlate With ECM Structure and Alignment.

ABSTRACT:

Purpose

To assess keratocyte backscattering, alignment, morphology, and connectivity in vivo following a full-thickness corneal injury using the Heidelberg Retina Tomograph Rostock Cornea Module (HRT-RCM), and to correlate these findings with en bloc three-dimensional (3-D) confocal fluorescence and second harmonic generation (SHG) imaging.

Methods

Rabbit corneas were scanned in vivo both before and 3, 7, 14, and 28 days after transcorneal freeze injury (FI), which damages all corneal cell layers. Corneal tissue was also fixed and labeled for f-actin and nuclei en bloc, and imaged using 3-D confocal fluorescence microscopy and SHG imaging.

Results

Using the modified HRT-RCM, full-thickness scans of all cell layers were consistently obtained. Following FI, stromal cell

SUBMITTER: Petroll WM 

PROVIDER: S-EPMC4645812 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature