Microbial Disruption of Autophagy Alters Expression of the RISC Component AGO2, a Critical Regulator of the miRNA Silencing Pathway.

ABSTRACT:

Background

Autophagy is implicated in Crohn's disease (CD) pathogenesis. Recent evidence suggests autophagy regulates the microRNA (miRNA)-induced silencing complex (miRISC). Therefore, autophagy may play a novel role in CD by regulating expression of miRISC, thereby altering miRNA silencing. As microbes associated with CD can alter autophagy, we hypothesized that microbial disruption of autophagy affects the critical miRISC component AGO2.

Methods

AGO2 expression was assessed in epithelial and immune cells, and intestinal organoids with disrupted autophagy. Microarray technology was used to determine the expression of downstream miRNAs in cells with defective autophagy.

Results

Increased AGO2 was detected in autophagy-deficient ATG5-/- and ATG16-/- mouse embryonic fi

SUBMITTER: Sibony M 

PROVIDER: S-EPMC4645954 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature