The cell wall amidase AmiB is essential for Pseudomonas aeruginosa cell division, drug resistance and viability.

ABSTRACT: The physiological function of cell wall amidases has been investigated in several proteobacterial species. In all cases, they have been implicated in the cleavage of cell wall material synthesized by the cytokinetic ring. Although typically non-essential, this activity is critical for daughter cell separation and outer membrane invagination during division. In Escherichia coli, proteins with LytM domains also participate in cell separation by stimulating amidase activity. Here, we investigated the function of amidases and LytM proteins in the opportunistic pathogen Pseudomonas aeruginosa. In agreement with studies in other organisms, (Pa) AmiB and three LytM proteins were found to play crucial roles in P. aeruginosa cell separation, envelope integrity and antibiotic resistance. Importantly

SUBMITTER: Yakhnina AA 

PROVIDER: S-EPMC4646093 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature