Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing.

ABSTRACT: Knowledge of the fine location of neutralizing and non-neutralizing epitopes on human pathogens affords a better understanding of the structural basis of antibody efficacy, which will expedite rational design of vaccines, prophylactics, and therapeutics. However, full utilization of the wealth of information from single cell techniques and antibody repertoire sequencing awaits the development of a high throughput, inexpensive method to map the conformational epitopes for antibody-antigen interactions. Here we show such an approach that combines comprehensive mutagenesis, cell surface display, and DNA deep sequencing. We develop analytical equations to identify epitope positions and show the method effectiveness by mapping the fine epitope for different antibodies targeting TNF, pertussis t

SUBMITTER: Kowalsky CA 

PROVIDER: S-EPMC4646304 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature