Galactomannan and Zymosan Block the Epinephrine-Induced Particle Transport in Tracheal Epithelium.

ABSTRACT:

Background

Ciliary beating by respiratory epithelial cells continuously purges pathogens from the lower airways. Here we investigated the effect of the fungal cell wall polysaccharides Galactomannan (GM) and Zymosan (Zym) on the adrenergic activated particle transport velocity (PTV) of tracheal epithelium.

Methods

Experiments were performed using tracheae isolated from male C57BL/6J mice. Transport velocity of the cilia bearing epithelial cells was measured by analysing recorded image sequences. Generation of reactive oxygen species (ROS) were determined using Amplex Red reagents. PCR experiments were performed on isolated tracheal epithelium to identify adrenergic receptor mRNA.

Results

The adrenergic receptors α1D, α2A, β1 and β2 have been identified in isolated tra

SUBMITTER: Weiterer S 

PROVIDER: S-EPMC4646458 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature