Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.

ABSTRACT: Autofocusing is a routine technique in redressing focus drift that occurs in time-lapse microscopic image acquisition. To date, most automatic microscopes are designed on the distance detection scheme to fulfill the autofocusing operation, which may suffer from the low contrast of the reflected signal due to the refractive index mismatch at the water/glass interface. To achieve high autofocusing speed with minimal motion artifacts, we developed a compact multi-band fluorescent microscope with an electrically tunable lens (ETL) device for autofocusing. A modified searching algorithm based on equidistant scanning and curve fitting is proposed, which no longer requires a single-peak focus curve and then efficiently restrains the impact of external disturbance. This technique enables us to ach

SUBMITTER: Wang Z 

PROVIDER: S-EPMC4646545 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature