Spatiotemporal quantification of subcellular ATP levels in a single HeLa cell during changes in morphology.

ABSTRACT: The quantitative relationship between change in cell shape and ATP consumption is an unsolved problem in cell biology. In this study, a simultaneous imaging and image processing analysis allowed us to observe and quantify these relationships under physiological conditions, for the first time. We focused on two marginal regions of cells: the microtubule-rich 'lamella' and the actin-rich 'peripheral structure'. Simultaneous imaging and correlation analysis revealed that microtubule dynamics cause lamellar shape change accompanying an increase in ATP level. Also, image processing and spatiotemporal quantification enabled to visualize a chronological change of the relationships between the protrusion length and ATP levels, and it suggested they are influencing each other. Furthermore, inhibiti

SUBMITTER: Suzuki R 

PROVIDER: S-EPMC4647183 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature