Interleukin-8 is a key mediator of FKBP51-induced melanoma growth, angiogenesis and metastasis.

ABSTRACT:

Background

FKBP51 is overexpressed in melanoma and impacts tumour cell properties. However, its comprehensive role in melanoma pathogenesis and underlying mechanism(s) remain elusive.

Methods

FKBP51 was stably silenced in aggressive melanoma cell lines and its effect examined in vitro and in mouse model. Histological/immunohistochemical analyses were performed to confirm metastasis, angiogenesis and neutrophil infiltration. Gene expression was analyzed by qRT-PCR, immunoblot and/or ELISA. NF-κB transcriptional activity and promoter binding were monitored by luciferase-based promoter-reporter and ChIP assays, respectively. Interleukin (IL)-8 inhibition was achieved by gene silencing or neutralising-antibody treatment.

Results

FKBP51 silencing reduced melanoma growth, m

SUBMITTER: Srivastava SK 

PROVIDER: S-EPMC4647250 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature