Population genetic structure and natural selection of apical membrane antigen-1 in Plasmodium vivax Korean isolates.

ABSTRACT:

Background

Plasmodium vivax apical membrane antigen-1 (PvAMA-1) is a leading candidate antigen for blood stage malaria vaccine. However, antigenic variation is a major obstacle in the development of an effective vaccine based on this antigen. In this study, the genetic structure and the effect of natural selection of PvAMA-1 among Korean P. vivax isolates were analysed.

Methods

Blood samples were collected from 66 Korean patients with vivax malaria. The entire PvAMA-1 gene was amplified by polymerase chain reaction and cloned into a TA cloning vector. The PvAMA-1 sequence of each isolate was sequenced and the polymorphic characteristics and effect of natural selection were analysed using the DNASTAR, MEGA4, and DnaSP programs.

Results

Thirty haplotypes of PvAMA-1, whi

SUBMITTER: Kang JM 

PROVIDER: S-EPMC4647566 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature