Dormant non-culturable Mycobacterium tuberculosis retains stable low-abundant mRNA.

ABSTRACT:

Background

Dormant Mycobacterium tuberculosis bacilli are believed to play an important role in latent tuberculosis infection. Previously, we have demonstrated that cultivation of M. tuberculosis in K(+)-deficient medium resulted in generation of dormant cells. These bacilli were non-culturable on solid media (a key feature of dormant M. tuberculosis in vivo) and characterized by low metabolism and tolerance to anti-tuberculosis drugs. The dormant bacteria demonstrated a high potential to reactivation after K(+) reintroduction even after prolonged persistence under rifampicin. In this work, we studied the transcriptome and stability of transcripts in persisting dormant bacilli under arrest of mRNA de novo synthesis.

Results

RNA-seq-based analysis of the dormant non-culturabl

SUBMITTER: Ignatov DV 

PROVIDER: S-EPMC4647672 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature