EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions.

ABSTRACT:

Background

FUS (TLS) and EWS (EWSR1) belong to the FET-protein family of RNA and DNA binding proteins. FUS and EWS are structurally and functionally related and participate in transcriptional regulation and RNA processing. FUS and EWS are identified in translocation generated cancer fusion proteins and involved in the human neurological diseases amyotrophic lateral sclerosis and fronto-temporal lobar degeneration.

Results

To determine the gene regulatory functions of FUS and EWS at the level of chromatin, we have performed chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Our results show that FUS and EWS bind to a subset of actively transcribed genes, that binding often is downstream the poly(A)-signal, and that binding overlaps with RNA polym

SUBMITTER: Luo Y 

PROVIDER: S-EPMC4647676 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature