Construction of therapeutically relevant human prostate epithelial fate map by utilising miRNA and mRNA microarray expression data.

ABSTRACT:

Background

Objective identification of key miRNAs from transcriptomic data is difficult owing to the inherent inconsistencies within miRNA target-prediction algorithms and the promiscuous nature of miRNA-mRNA target relationship.

Methods

An integrated database of miRNAs and their 'relevant' mRNA targets was generated from validated miRNA and mRNA microarray data sets generated from patient-derived prostate epithelial normal and cancer stem-like cells (SCs) and committed basal (CB) cells. The effect of miR-542-5p inhibition was studied to provide proof-of-principle for database utility.

Results

Integration of miRNA-mRNA databases showed that signalling pathways and processes can be regulated by a single or relatively few miRNAs, for example, DNA repair/Notch pathway by

SUBMITTER: Rane JK 

PROVIDER: S-EPMC4647689 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature