Deep sequencing of the uterine immune response to bacteria during the equine oestrous cycle.

ABSTRACT:

Background

The steroid hormone environment in healthy horses seems to have a significant impact on the efficiency of their uterine immune response. The objective of this study was to characterize the changes in gene expression in the equine endometrium in response to the introduction of bacterial pathogens and the influence of steroid hormone concentrations on this expression.

Methods

Endometrial biopsies were collected from five horses before and 3 h after the inoculation of Escherichia coli once in oestrus (follicle >35 mm in diameter) and once in dioestrus (5 days after ovulation) and analysed using high-throughput RNA sequencing techniques (RNA-Seq).

Results

Comparison between time points revealed that 2422 genes were expressed at significantly higher levels and 2

SUBMITTER: Marth CD 

PROVIDER: S-EPMC4647707 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature